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Polygonum cuspidatum

Planta Med. 2010 Jan 28.

Anti-HIV Activities of the Compounds Isolated from Polygonum cuspidatum and Polygonum multiflorum.

Lin HW, Sun MX, Wang YH, Yang LM, Yang YR, Huang N, Xuan LJ, Xu YM, Bai DL, Zheng YT, Xiao K.

Lab of Toxicology & Pharmacology, Faculty of Naval Medicine, The Second Military Medical University, Shanghai, P. R. China.

The 70 % EtOH extract of POLYGONUM CUSPIDATUM showed inhibitory action against HIV-1-induced syncytium formation at non-cytotoxic concentrations IN VITRO with a 50 % effective concentration (EC (50)) of 13.94 +/- 3.41 micro g/ml. Through bio activity-guided fractionation, 20 phenolic compounds, including eight stilbenoids, were isolated from the roots of POLYGONUM CUSPIDATUM, and their anti-HIV-1 activities were evaluated. Results showed that compounds 1, 13, 14, and 16 demonstrated fairly strong antiviral activity against HIV-1-induced cytopathic effects in C8166 lymphocytes at non-cytotoxic concentrations, with EC (50) values of 4.37 +/- 1.96 micro g/ml, 19.97 +/- 5.09, 14.4 +/- 1.34 micro g/mL, and 11.29 +/- 6.26 microg/mL and therapeutic index (TI) values of 8.12, > 10.02, > 13.89, and > 17.71, respectively. Other compounds showed either weak or no effects. Compound 6 also showed weak inhibition (153.42 +/- 19.25 microg/mL); however, it possesses very good water solubility and showed almost no cytotoxicity (> 2000 microg/mL), therefore achieving a fairly good TI (13.04). The activities of the two compounds ( 3 and 18) from POLYGONUM MULTIFLORUM were also assayed. The relationship between molecular structures and their bioactivities was also discussed.

Source: PubMed

Antiviral Res. 2005 Apr;66(1):29-34.

Ethanol extract of Polygonum cuspidatum inhibits hepatitis B virus in a stable HBV-producing cell line.

Chang JS, Liu HW, Wang KC, Chen MC, Chiang LC, Hua YC, Lin CC.

Graduate Institute of Medicine, College of Medicine , Kaohsiung Medical University , 100 Shih-Chuan First Road , Kaohsiung 807, Taiwan .

Chronic hepatitis B virus (HBV) infection is endemic in Asia and its consequences are among the major public health problems in the world. Unfortunately, the therapeutic efficacies of present strategies are still unsatisfactory with a major concern about viral mutation. In search of effective antiviral agent, we examined the efficacy of extracts of Polygonum cuspidatum Sieb. et Zucc. (P. cuspidatum) against HBV in HepG2 2.2.15 cells by quantitative real time polymerase chain reaction. The expressions of viral antigens, HBeAg and HBsAg, were also determined by enzyme linked immunosorbent assay. The ethanol extract of P. cuspidatum could inhibit dose-dependently the production of HBV (p<0.0001) with an effective minimal dosage of 10 microg/ml. The water extract of P. cuspidatum might also inhibit the production of HBV at a higher dosage. The expression of HBsAg was significantly increased by both ethanol extract and water extract of P. cuspidatum dose-dependently (p<0.0001) and time-dependently (p<0.0001). Higher dose of water extract of P. cuspidatum (30 microg/ml) could inhibit the expression of HBeAg (p<0.05). The extract of P. cuspidatum might contain compounds that would contribute to the control of HBV infection in the future. However, its promoting effect on the expression of HBsAg and its cytotoxicity should be monitored. Further purification of the active compounds, identification and modification of their structures to improve the efficacy and decrease the cytotoxicity are required.

Source: PubMed

Biol Res. 2007;40(1):13-21. Epub 2007 Jul 19.

Antioxidant activity of extract from Polygonum cuspidatum.

Hsu CY, Chan YP, Chang J.

Department of Life Science, Chang Gung University , Tao-Yuan , Taiwan , China .

Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum cuspidatum Sieb. et Zuce has antioxidant activity is unknown. In this study, dried roots of Polygonum cuspidatum were extracted by ethanol and the extract was lyophilized. Free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays were employed to study antioxidant activities. The results indicate that the IC50 value oí Polygonum cuspidatum extract is 110 microg/ml in free radical scavenging assays, 3.2 microg/ml in superoxide radical scavenging assays, and 8 microg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum cuspidatum extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 641.1 +/- 42.6 mg/g and 62.3 +/- 6.0 mg/g. The results indicate that Polygonum cuspidatum extract clearly has antioxidant effects .

Source: PubMed